一、Human FXR ELISA 試劑盒的產(chǎn)品簡述：

       ELISA 品  牌：USCN

       ELISA 貨  號(hào)：072042h 

       ELISA英文名：Human FXR ELISA Kit

       ELISA中文名：人的法尼酯X受體，FXR ELISAkit

二、  人法尼酯X受體ELISA kit的技術(shù)參數(shù)：

      1. 檢測范圍： 

      2. 靈 敏 度：

      3. 適用標(biāo)本：

      4. 保存條件：2-8℃

       5. 有 效 期：6個(gè)月

三、人FXR ELISA試劑盒的產(chǎn)品用途：

      產(chǎn)品用途：酶聯(lián)免疫實(shí)驗(yàn)(ELISA實(shí)驗(yàn))

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ELISA實(shí)驗(yàn)前，血清、血漿、組織、細(xì)胞培養(yǎng)上清等樣品的采集及處理 

 Serum- Use a serum separator tube and allow samples to clot for two hours at room temperature or overnight at 4oC before centrifugation for 20 minutes at approximately 1000×g. Assay freshly prepared serum immediately or store samples in aliquot at -20oC or -80oC for later use. Avoid repeated freeze/thaw cycles.

Plasma- Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 15 minutes at  000×g at 2 - 8oC within 30 minutes of collection. Remove plasma and assay immediately or store samples in aliquot at -20oC or -80oC for later use. Avoid repeated freeze/thaw cycles.

Tissue homogenates - The preparation of tissue homogenates will vary depending upon tissue type. For this assay, tissues were rinsed in ice-cold PBS(0.02mol/L,pH 7.0-7.2) to remove excess blood thoroughly and weighed before homogenization. Minced the tissues to small pieces and homogenized them in 5-10 mL of PBS with a glass homogenizer on ice(Micro Tissue Grinders woks, too). The resulting suspension was sonicated with an ultrasonic cell disrupter or subjected to two freeze-thaw cycles to further break the cell membranes. After that, the homogenates were centrifugated for 5 minutes at 5000×g. Remove the supernate and assay immediately or aliquot and store at ≤-20oC.

Cell culture supernates - Centrifuge samples for 20 minutes at 1000×g. Remove particulates and assay immediately or store samples in aliquot at -20oC or -80oC for later use. Avoid repeated freeze/thaw cycles.